Rapid, competitive enzymoimmunoassay for albumin in urine.
نویسندگان
چکیده
In this solid-phase competitive enzymoimmunoassay for albumin in human urine, antiserum to human serum albumin labeled with horseradish peroxidase (EC 1.11.1.7) is incubated with solid-phase-bound human serum albumin in the presence of sample or standard. Results obtained correlate well (r = 0.96) with those of an established fluoroimmunoassay. The present assay covers the range 0.9 to 200 mg/L and can be performed within 1 h. These characteristics, together with the simplicity of the assay protocol, make it very useful for monitoring low concentrations of albumin in urine. Detection of such minimal albuminuria allows initiation of therapy that may prevent development of clinical proteinuria and associated diabetic nephropathy.
منابع مشابه
A competitive immunoenzymometric assay for albumin in urine.
I describe a competitive immunoenzymometric assay for urinary albumin, with use of an immobilized albumin-thyroglobulin conjugate and an affinity-purified antibody. The assay is rapid, easy to perform, has a range of 0.5 to 78 mg/L, and is ideally suited for screening purposes. Higher concentrations may be assayed by further dilution of the urine sample. Results compare favorably with those of ...
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عنوان ژورنال:
- Clinical chemistry
دوره 32 4 شماره
صفحات -
تاریخ انتشار 1986